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Evaluation of the level of interleukin-5 (IL-5) in mice sera post vaccination with Beta-propiolactone ( β -PL) and Ascorbic acid (AA) inactivated rabies vaccines relative to time using ELISA. DPV: days after vaccination; DPC: days after challenge. ∗ : statistically significant difference.

Journal: BioMed Research International

Article Title: Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity: In Vitro Study

doi: 10.1155/2019/4518163

Figure Lengend Snippet: Evaluation of the level of interleukin-5 (IL-5) in mice sera post vaccination with Beta-propiolactone ( β -PL) and Ascorbic acid (AA) inactivated rabies vaccines relative to time using ELISA. DPV: days after vaccination; DPC: days after challenge. ∗ : statistically significant difference.

Article Snippet: High-protein-binding ELISA plates were coated with anti-mouse Interferon gamma (IFN- γ ) and anti-mouse Interleukin-5 (IL-5) (Biosciences Pharmingen, USA) monoclonal antibodies (100 μ l /well; 2.5 μ g/ml) against IFN- γ and IL-5 cytokines, respectively, for at least 18 h at 4°C in a carbonate/bicarbonate coating buffer (1 L of H 2 O containing 0.07 M Na 2 CO 3 and 0.173 M NaHCO 3 , pH 9.6).

Techniques: Enzyme-linked Immunosorbent Assay